منابع مشابه
Glycosyl moiety of the lima bean lectin.
Pronase digestion of the lima bean lectin (Phaseolus lunatus (alpha-D-GalNAc greater than alpha-D-Gal)) afforded a glycopeptide of molecular weight 1380. Amino acid and carbohydrate analysis showed the glycopeptide to contain fucose, mannose, glucosamine, and aspartic acid in the molar ratios, 0.8:4.4:1.9:1.0. Methylation analysis, Smith degradation, and sequential degradation by specific glyco...
متن کاملAntigenic and calcium binding properties of a Peptide containing the essential cysteine in lima bean lectin.
Polyclonal antisera were raised against a peptide containing the cysteine residue required for carbohydrate binding activity in the lima bean lectin. The antisera were tested for cross-reactivity with (a) synthetic peptide analogs to the essential cysteine containing peptide, (b) proteolytic digests of related lectins, (c) native lectins. The antisera were specifically inhibited from binding to...
متن کاملEffect of carbohydrate and metal ion binding on the reactivity of the essential thiol groups of lima bean lectin.
A free sulfhydryl group previously has been shown to be required for carbohydrate binding to the lectin from lima bean (Phaseolus lunatus) (Gould, N. R. and Scheinberg, S. L. (1970) Arch. Biochem. Biophys. 141, 607-613). Modification of this group by sulfhydryl reagents was specifically inhibited by D-GalNAc. We have further examined the reactivity of sulfhydryl groups in lima bean lectin with ...
متن کاملThe sequence of a second member of the lima bean lectin gene family and the expression and characterization of recombinant lectin in Escherichia coli.
The lima bean lectin recognizes terminal alpha-D-GalNAc groups and agglutinates human type A erythrocytes. We have cloned a portion of the gene encoding the alpha subunit of the lima bean lectin. The clone was obtained using the polymerase chain reaction and verified from a genomic clone encoding the mature protein of 253 amino acids. The deduced amino acid sequence has significant overall homo...
متن کاملEvidence for Genetic Restriction of Recombination in the Lima Bean.
is some evidence from studies of related species of plants that low T E a E a frequency is associated with outcrossing and high chiasma frequency is associated with selfing (reviews in GRANT 1958; BODMER and PARSONS 1962). From this it has been inferred that crossover rates have been adjusted by natural selection toward high values in selfing species to compensate for the restrictions imposed o...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)66925-5